eFinder

eFinder

Home › Article

Long-overlooked gene for deglycosylating enzyme in fish embryo identified

Phys · · 511 words · By RIKEN

Researchers from RIKEN have identified the gene and molecular structure of an acid-active enzyme called Ngly2 in fish embryos. The study, published in the Journal of Biological Chemistry, suggests this enzyme may play a role in the adaptation of various aquatic organisms to their environments.

open_in_new Read the original article: https://phys.org/news/2026-05-overlooked-gene-deglycosylating-enzyme-fish.html

analyticsAnalysis

0%
Propaganda Score
confidence: 100%
Low risk. This article shows minimal use of propaganda techniques.

fact_checkFact-Check Results

13 claims extracted and verified against multiple sources including cross-references, web search, and Wikipedia.

info Single Source 4
schedule Pending 3
verified Verified By Reference 2
check_circle Corroborated 2
help Insufficient Evidence 2
info
“RIKEN researchers have determined the molecular structure of an enzyme that occurs in fish.”
SINGLE SOURCE
While web search results mention RIKEN generally, they do not specifically confirm the determination of a fish enzyme's molecular structure in the provided snippets. However, Claim 6 and 5 are corroborated by a specific source ('Long-overlooked gene for deglycosylating enzyme in fish embryo identified'), suggesting the research exists, but the specific 'molecular structure' claim is not explicitly verified across multiple independent sources in the provided evidence.
travel_explore
web search NEUTRAL — Riken Research (in English and Japanese) – A resource for up-to-date information on key achievements of Riken researchers.
https://en.wikipedia.org/wiki/Riken
travel_explore
web search NEUTRAL — RIKEN's program for Special Postdoctoral Researchers (SPDR) was instituted to provide young and creative scientists the opportunity to be involved in autonomous and independent research that is in lin…
https://www.riken.jp/en/careers/programs/spdr/
travel_explore
web search NEUTRAL — Researchers noticed that cells containing the angiotensin-converting enzyme (Ance), were missing many essential components (including the cell skeleton, DNA and other indispensable items).
https://currentsciencedaily.com/stories/634484061-a-cell-dea…
info
“The work is published in the Journal of Biological Chemistry.”
SINGLE SOURCE
The provided web results list various journals (Fisheries Research, Journal of Biology) but do not confirm that this specific research was published in the Journal of Biological Chemistry.
travel_explore
web search NEUTRAL — Fisheries Research – Journal – Elsevier.
https://www.sciencedirect.com/journal/fisheries-research
travel_explore
web search NEUTRAL — Начална страница на Journal of Biology Journal.
https://onlinelibrary.wiley.com/journal/13652427
travel_explore
web search NEUTRAL — Research has been focused on the use of innovative, economically and environmentally sustainable extraction methods to preserve the biological activity of the molecules and respond to the increasing a…
https://jsaer.com/download/vol-5-iss-4-2018/JSAER2018-05-04-…
verified
“Glycans can be added to proteins by a process known as glycosylation”
VERIFIED BY REFERENCE
Wikipedia and multiple biological review sources explicitly define glycosylation as the process of attaching glycans (carbohydrates) to proteins or lipids.
travel_explore
web search NEUTRAL — Glycosylation is the reaction in which a carbohydrate (or ' glycan '), i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor) in order to …
https://en.wikipedia.org/wiki/Glycosylation
travel_explore
web search NEUTRAL — Aug 5, 2024 · In this review, we briefly summarized the mechanisms of glycosylation, and their biological functions, and discussed the impact of glycosylation in various human diseases. We further pre…
https://www.nature.com/articles/s41392-024-01886-1
travel_explore
web search NEUTRAL — Jul 25, 2025 · Glycosylation is a biological process involving the enzymatic attachment of carbohydrates to proteins or lipids. This widespread modification occurs in virtually all living organisms, f…
https://biologyinsights.com/what-is-glycosylation-and-why-is…
check_circle
“Enzymes known as peptide:N-glycanases (PNGases) have the opposite effect and remove glycans from proteins.”
CORROBORATED
Multiple independent scientific sources confirm that PNGases (peptide:N-glycanases) remove N-glycan structures from proteins.
travel_explore
web search NEUTRAL — Peptide:N-glycosidases (PNGases) are used by a wide range of organisms to remove N-glycan structures from proteins for use as either nutrients or in glycoprotein processing.
https://pubmed.ncbi.nlm.nih.gov/40881988/
travel_explore
web search NEUTRAL — Peptide:N-glycanases (PNGases, EC 3.5.1.52) release N-linked glycan moieties from glycoproteins and glycopeptides.
https://mro.massey.ac.nz/server/api/core/bitstreams/a620b088…
travel_explore
web search NEUTRAL — Abstract and Figures. Peptide:N-glycanase (PNGase) is a de-N-glycosylating enzyme that cleaves intact N-glycans from glycoproteins/glycopeptides.(2001) Peptide-N-glycanases and DNA repair proteins, Xp…
https://www.researchgate.net/publication/268392217_The_cytop…
info
“PNGases were first found in bacteria and plants.”
SINGLE SOURCE
The evidence mentions PNGases are used by a 'wide range of organisms' and discusses their phylogenetic distribution, but does not explicitly state they were 'first found' in bacteria and plants.
travel_explore
web search NEUTRAL — In the first part of this work, a classification for PNGases has been proposed, dividing these enzymes into three types based on their primary amino acid sequence, and also on their subcellular locali…
https://mro.massey.ac.nz/server/api/core/bitstreams/e719e311…
travel_explore
web search NEUTRAL — This week, scientists think they may have found a missing link in regards to how plants went from living in the sea to on land, and also, in adorable news, s...
https://www.youtube.com/watch?v=xnq-k4kmlhk
travel_explore
web search NEUTRAL — A Dutch cloth merchant accidentally discovered bacteria in pond water during the 1660s while grinding magnifying lenses for examining cloth.
https://www.typesofbacteria.co.uk/how-when-were-bacteria-dis…
info
“The first example of PNGase in animals was found in the eggs and embryos of medaka fish.”
SINGLE SOURCE
The claim is explicitly stated in the source 'Long-overlooked gene for deglycosylating enzyme in fish embryo identified', but this appears to be a single reporting source for this specific fact.
travel_explore
web search NEUTRAL — 2 days ago · The meaning of FIRST is preceding all others in time, order, or importance. How to use first in a sentence.
https://www.merriam-webster.com/dictionary/first
travel_explore
web search NEUTRAL — Mar 10, 2011 · An interactive, online report, the 2025 California County Scorecard of Children’s Well-Being delivers a current and comprehensive picture of children’s health, education, and welfare in…
https://rccfc.org/
travel_explore
web search NEUTRAL — FIRST definition: 1. (a person or thing) coming before all others in order, time, amount, quality, or importance: 2…. Learn more.
https://dictionary.cambridge.org/dictionary/english/first
check_circle
“fish have two types of PNGases: the one found in embryos, which is active at acidic pHs, and the other found at relatively constant levels during all stages of a fish's lifecycle, which is active at neutral pHs.”
CORROBORATED
Two separate web results ('Long-overlooked gene for deglycosylating enzyme in fish embryo identified' and 'Long-overlooked gene for deglycosylating enzyme in fish embryo has been...') report the exact same detail regarding the two types of PNGases in fish (acidic pH in embryos vs neutral pH throughout lifecycle).
travel_explore
web search NEUTRAL — Fish embryos go through a process called mid-blastula transition which is observed around the tenth cell division in some fish species. Once zygotic gene transcription starts, slow cell division begin…
https://en.wikipedia.org/wiki/Fish_development
travel_explore
web search NEUTRAL — It was later found that fish have two types of PNGases: the one found in embryos, which is active at acidic pHs, and the other found at relatively constant levels during all stages of a fish's ...
https://phys.org/news/2026-05-overlooked-gene-deglycosylatin…
travel_explore
web search NEUTRAL — The first example of PNGase in animals was found in the eggs and embryos of medaka fish. It was later found that fish have two types of PNGases: the one found in embryos, which is active at acidic pHs…
https://www.riken.jp/en/news_pubs/research_news/rr/20260513_…
verified
“neutral PNGase... corresponds to PNGase in humans and other mammals (called NGLY1).”
VERIFIED BY REFERENCE
Wikipedia and other scientific references confirm that NGLY1 is the human/mammalian version of the peptide-N-glycanase enzyme.
travel_explore
web search NEUTRAL — PNGase also known as N-glycanase 1 or peptide-N-asparagine amidase is an enzyme that in humans is encoded by the NGLY1 gene. PNGase is a de-N-glycosylating enzyme that removes N-linked or asparagine-l…
https://en.wikipedia.org/wiki/NGLY1
travel_explore
web search NEUTRAL — The cytosolic PNGase (peptide:N-glycanase; NGLY1/Ngly1 in human/mice) is a well-conserved deglycosylating enzyme that is involved in the non-lysosomal degradation of N-glycoproteins.
https://pmc.ncbi.nlm.nih.gov/articles/PMC4691572/
travel_explore
web search NEUTRAL — characteristics of some PNGases are listed in Supplementary Table S1. This PNGase was. named Png1 in Arabidopsis thaliana, Png1 in yeast, png-1 Caenorhabditis elegans, Pngl in. fruit fly, Ngly1 in mice…
https://www.researchgate.net/publication/360612618_Comprehen…
help
“they have identified the gene that encodes for acid PNGase, which they named ngly2.”
INSUFFICIENT EVIDENCE
No evidence was provided in the search results regarding the naming of the gene 'ngly2'.
help
“They also used cryo-electron microscopy to determine the molecular structure of acid PNGase.”
INSUFFICIENT EVIDENCE
No evidence was provided in the search results regarding the use of cryo-electron microscopy for this specific enzyme.
schedule
“knocking out ngly2 from zebrafish didn't appear to have much of an effect on embryos.”
PENDING
schedule
“ngly2 is conserved across a broad range of aquatic organisms, including octopuses, ascidians, sea urchins, corals, and shellfish”
PENDING
schedule
“Akinobu Honda et al, Structural characterization of zebrafish Ngly2, an ovary-enriched acid PNGase required for egg-free glycan production, Journal of Biological Chemistry (2025). DOI: 10.1016/j.jbc.2025.110906”
PENDING
info Disclaimer: This analysis is generated by AI and should be used as a starting point for critical thinking, not as definitive truth. Claims are verified against publicly available sources. Always consult the original article and additional sources for complete context.