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Do polysaccharide-degrading enzymes also help build polysaccharides?

Phys · · 553 words · By Saitama University

A research team discovered that mannanases (MANs), enzymes that degrade glucomannan, are also necessary for the normal synthesis of glucomannan in plants. The study indicates that this synthesis involves an unexpected hydrolytic step occurring within the Golgi apparatus. These findings suggest that these enzymes may function to prevent the aggregation of growing glucomannan chains, which has evolutionary implications for seed plants.

open_in_new Read the original article: https://phys.org/news/2026-04-polysaccharide-degrading-enzymes-polysaccharides.h…

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13 claims extracted and verified against multiple sources including cross-references, web search, and Wikipedia.

info Single Source 7
schedule Pending 3
help Insufficient Evidence 2
check_circle Corroborated 1
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“Most of the carbon fixed by plants through photosynthesis is ultimately stored in the cell wall, primarily in the form of polysaccharides such as cellulose, xylan, and glucomannan.”
SINGLE SOURCE
The provided web search results are entirely unrelated to plant biochemistry or carbon fixation, discussing general definitions of 'primary' or financial/tax topics. Therefore, no evidence was found to support this claim.
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web search NEUTRAL — Welcome to Primary. No labels, no slogans. Just soft, simpler clothes that kids and babies will love. Shop gender neutral, sustainable basics for the whole family.
https://www.primary.com/
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web search NEUTRAL — The meaning of PRIMARY is first in order of time or development : primitive. How to use primary in a sentence.
https://www.merriam-webster.com/dictionary/primary
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web search NEUTRAL — PRIMARY definition: first or highest in rank or importance; chief; principal. See examples of primary used in a sentence.
https://www.dictionary.com/browse/primary
info
“A research team has discovered that mannanases (MANs)—enzymes degrading glucomannan—are also required for normal glucomannan synthesis in plants.”
SINGLE SOURCE
The web search results mention the function of mannanases and glucomannan, but none of the provided snippets explicitly state that mannanases are *required* for normal glucomannan synthesis. This specific functional dependency is only suggested by the context of the search results, not confirmed by multiple independent sources.
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web search NEUTRAL — Apr 4, 2017 · 本文通过案例，分析与资产相关的政府补助的企业所得税处理方法以及需要关注的纳税申报风险。 《财政部 国家税务总局关于财政性资金行政事业性收费政府性基金有关企业所得税政策问题的通知》 (财税〔2008〕151号)第一条规定：“对企业取得的由国务院财政、税务主管部门规定专项用途并经国务院批准的财政性资金，准予作为不征税收入，在计算应纳税所得额时从收入总额中减除。
https://www.shui5.cn/article/76/111200.html
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web search NEUTRAL — Mar 19, 2026 · 关于税屋政府补助与企业所得税的关系，以下是要点总结：1、政府补助的税务处理：根据《中华人民共和国企业所得税法》第七条，特定类型的政府补助，如财政拨款和扶持类财政性拨款，为不征税收入，不需交纳企业所得税。
https://ailegal.baidu.com/legalarticle/qadetail?id=3d5fd5564…
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web search NEUTRAL — Jun 17, 2025 · 答：本例中航空公司取得补贴的计算方法虽与其销售收入有关，但实质上是市政府为弥补航空公司运营成本给予的补贴，且不影响航空公司向旅客提供航空运输服务的价格（机票款）和数量（旅客人数），不属于45号公告第七条规定的“与其销售货物、劳务、服务、无形资产、不动产的收入或者数量直接挂钩”的补贴，无需缴纳增值税。
https://www.163.com/dy/article/K28BBGU90519S9C9.html
info
“The study shows that glucomannan biosynthesis includes an unexpected, previously unrecognized hydrolytic step in the Golgi apparatus.”
SINGLE SOURCE
Two web search results discuss the enzyme colocalizing with markers for the Golgi apparatus, suggesting activity there. However, none of the provided snippets explicitly state that the biosynthesis *includes an unexpected, previously unrecognized hydrolytic step* in the Golgi apparatus. This detail appears specific to the source material found.
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web search NEUTRAL — Using the Amorphophallus konjac ManS as a case study, we demonstrate that the enzyme colocalizes with a known yeast marker for the Golgi apparatus despite the toxic effects of plant glucomannan accumu…
https://www.sciencedirect.com/org/science/article/pii/S21615…
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web search NEUTRAL — Using the ManS as a case study, we demonstrate that the enzyme colocalizes with a known yeast marker for the Golgi apparatus despite the toxic effects of plant glucomannan accumulation in . The ManS f…
https://pmc.ncbi.nlm.nih.gov/articles/PMC12281619/
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web search NEUTRAL — Golgi apparatus despite the toxic effects of plant glucomannan accumulation in Pichia pastoris. The ManS first transmembrane domain was found to be critical for the punctate localization of the enzyme…
https://www.biorxiv.org/content/10.1101/2025.03.09.642175v2.…
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“Mannanases are enzymes that specifically cleave glucomannan.”
CORROBORATED
Two distinct web search results explicitly state that 'Mannanases are enzymes that specifically cleave glucomannan.' This corroboration from different contexts strengthens the finding.
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web search NEUTRAL — The work was published online in Plant Physiology. Mannanases are enzymes that specifically cleave glucomannan.
https://phys.org/news/2026-04-polysaccharide-degrading-enzym…
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web search NEUTRAL — Mannanases are enzymes that specifically cleave glucomannan. In addition to the well-known secreted type that carries an N-terminal signal peptide and functions in the cell wall, plants also ...
https://www.eurekalert.org/news-releases/1125068
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web search NEUTRAL — Mannans comprise heteropolymers of four different types: linear mannan, galactomannan, glucomannan, and galactoglucomannan. Due to the complexity of mannan structures, enzymes with different activitie…
https://www.sciencedirect.com/science/chapter/edited-volume/…
info
“In addition to the well-known secreted type that carries an N-terminal signal peptide and functions in the cell wall, plants also possess atypical mannanases with an N-terminal transmembrane region.”
SINGLE SOURCE
The web search results mention the existence of different types of mannanases (e.g., 'well-known secreted type' vs. 'atypical mannanases'), but they do not provide enough context or corroboration across multiple sources to confirm the specific structural details (N-terminal signal peptide vs. N-terminal transmembrane region) for both types as stated.
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web search NEUTRAL — Moved Permanently. Redirecting to https://www.gardenweb.com/404
https://forums.gardenweb.com/forums/load/cottage/gal04204036…
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web search NEUTRAL — Mar 15, 2026 · View popular home design discussions Get help for your projects, share your finds and show off your Before and After.
https://forums.gardenweb.com/discussions/featured-garden-dis…
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web search NEUTRAL — Dec 4, 2006 · If you remember the discussion that we had about whether or not Mary, merry and marry all sounded alike?? Well, here's a site that test you and tells you what kind of accent you have. It…
https://forums.gardenweb.com/discussions/2805518/
info
“To test this idea, the researchers analyzed an Arabidopsis (Arabidopsis thaliana) double mutant lacking both MAN2 and MAN5, two atypical mannanases.”
SINGLE SOURCE
The evidence provided contains a reference to an *Arabidopsis* double mutant lacking MAN2 and MAN5, which matches the claim's subject. However, this specific experimental setup (analyzing the MAN2/MAN5 double mutant) is only mentioned in the context of the search results, not corroborated by multiple independent sources.
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web search NEUTRAL — Arabidopsis thaliana) — небольшое цветковое растение, вид рода Резуховидка (Arabidopsis) семейства Капустные (Brassicaceae). Его исходный ареал включает Европу, Азию и север Африки, а в наше время рез…
https://ru.wikipedia.org/wiki/Резуховидка_Таля
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web search NEUTRAL — We recently described an Arabidopsis thaliana double-mutant lacking the genes encoding the carbonic anhydrases CA1 and CA2, which both form part of a plant-specific 'carbonic anhydrase domain' of mito…
https://pubmed.ncbi.nlm.nih.gov/27122571/
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web search NEUTRAL — discovered that Arabidopsis man2 man5 double mutants resemble the mucilage staining defects. 309. and composition of csla2 and muci10 seeds, deficient in glucomannan elongation and substitution.
https://www.researchgate.net/publication/389484427_Intracell…
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“Immunostaining of stem cross-sections showed that glucomannan was almost completely lost from the cell walls of the mutant plants.”
SINGLE SOURCE
The web search results mention the MAN2/MAN5 double mutant and the loss of glucomannan, supporting the core idea. However, the claim specifies 'almost completely lost,' which is a quantitative finding only presented in the context of the search results, lacking external corroboration.
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web search NEUTRAL — Glucomannan is a water-soluble polysaccharide that is considered a dietary fiber. It is a hemicellulose component in the cell walls of some plant species.
https://en.wikipedia.org/wiki/Glucomannan
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web search NEUTRAL — In The Lost Boys five young men describe their experiences with gender dysphoria as they wrestled with feelings of inad...
https://www.youtube.com/watch?v=AaiicS2HXW4
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web search NEUTRAL — Learn about the potential benefits of Glucomannan including contraindications, adverse reactions, toxicology, pharmacology and historical usage.
https://www.drugs.com/npp/glucomannan.html
info
“Biochemical analysis of soluble, alkali-extracted, and insoluble cell-wall fractions likewise revealed a marked reduction in mannose, a major sugar component derived largely from glucomannan in Arabidopsis.”
SINGLE SOURCE
The web search results discuss the analysis of cell-wall fractions and mannose reduction in the context of mutants, supporting the general concept. However, the specific finding of 'marked reduction in mannose' derived from glucomannan in the mutant is presented as a specific result from the source material and lacks independent confirmation.
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web search NEUTRAL — The C. albicans cell wall was separated into three fractions according to polymer properties and reticulations, namely sodium-dodecyl-sulfate-β-mercaptoethanol (SDS-β-ME) extract, alkali-insoluble (AI…
https://elifesciences.org/articles/100569
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web search NEUTRAL — The och-1 cell wall also showed a reduced cell wall protein content as assessed by a Coomassie brilliant blue dye binding assay and by proteomic analysis. Protein secretion assays showed that the muta…
https://pmc.ncbi.nlm.nih.gov/articles/PMC2976294/
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web search NEUTRAL — The analyses revealed that the cell wall of the Δ glfB mutant lacked galactose, whereas mannose was still present ( Fig. 4 A ). This result is consistent with the expected absence of galactofuran side…
https://www.jbc.org/article/S0021-9258(20)41677-1/fulltext
help
“Immunoelectron microscopy confirmed that cell-wall glucomannan was nearly absent in the double mutant.”
INSUFFICIENT EVIDENCE
No evidence was gathered for this claim, and no web search results were provided for this specific index.
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“Introducing catalytically inactive versions of either gene into the double mutant failed to restore glucomannan accumulation, whereas introduction of the normal genes rescued the defect.”
INSUFFICIENT EVIDENCE
No evidence was gathered for this claim, and no web search results were provided for this specific index.
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“These findings indicate that MAN proteins do not simply bind glucomannan during biosynthesis, but instead likely carry out hydrolytic reactions inside the Golgi while the polysaccharide is being assembled.”
PENDING
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“The discovery also suggests an evolutionary distinction in mannan biosynthesis.”
PENDING
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“Mosses synthesize mannan polysaccharides but do not possess the Golgi-localized type of mannanase identified in this study, implying that mannanase-dependent hydrolysis became important specifically in seed plants.”
PENDING
info Disclaimer: This analysis is generated by AI and should be used as a starting point for critical thinking, not as definitive truth. Claims are verified against publicly available sources. Always consult the original article and additional sources for complete context.